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Hdm2 (catalytic RING domain) (human), (recombinant) (GST-tag)
p53 is a much studied and complex multifunctional protein, which plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis, through its involvement in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for these processes. Activation and regulation of the p53 transcription pathway is controlled by a range of post-translational modifications including acetylation, phosphorylation and ubiquitinylation. In normal cells, p53 is maintained at a low level mainly through Hdm2-mediated ubiquitinylation and subsequent degradation by the proteasome. Hdm2 is a RING domain dependent ubiquitin E3 ligase that utilizes its C-terminal RING domain to promote not only p53 ubiquitinylation, predominantly at the C-terminus of p53, but also to target Hdm2 itself for auto-ubiquitinylation and subsequent degradation. The isolated Hdm2 C-terminal RING domain (residues 418-491) has been shown to be sufficient for both p53 and self-ubiquitinylation activity.
Product Specification
Alternative Name: Human double minute 2 protein
MW: ~35kDa
Source: Produced in E. coli.
UniProt ID: Q00987
Formulation: Liquid. In TBS, pH 7.5, containing 150mM sodium chloride and 1mM DTT.
Purity: ≥95% (SDS-PAGE)
Purity Detail: Purified by glutathione affinity chromatography.
Activity: Modifies p53 (Prod. No. BML-FW8820) in ubiquitinylation reactions containing Ube1 (Prod. No. BML-UW9410), UbcH5b (Prod. No. BML-UW9060) and energy regeneration solution.
Application: 1.in vitro generation of ubiquitinylated p53 for use in Ub-p53 related studies.
2.Investigation of the p53 ubiquitin modification process itself.
3.Ubiquitin modification of other known Hdm2 substrate proteins.
4.NEDD8 modification of p53.
Long Term Storage: -80°C |