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Product Specification
Alternative Name:HMOX1, Hsp32, Heat shock protein 32, HO-1
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 12-25 of human heme oxygenase 1 (HO-1) protein.
UniProt ID:P09601
Application:Immunohistochemistry (1:50 – 1:1'000)
Western Blot (1:1'000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human heme oxygenase 1.
Formulation:Liquid. Antiserum containing 10mM sodium azide.
Handling:Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month. Avoid freeze/thaw cycles.
Long Term Storage:-20°C
Miscellaneous/General:The rate limiting enzyme in heme catabolism, heme oxygenase, was first described in 1968 and was thought to be a species of cytochrome P450. However, subsequent work showed the enzyme to be a protein distinct from the P450 family and capable of catalysing the degradation of heme to biliverdin and iron, with the concomitant release of carbon monoxide. This enzyme activity is highly conserved throughout the Animal Kingdom and has been identified in algae and plants as well as in all higher species. Heme oxygenase-1 (HO-1) was first purified to homogeneity from rat liver and pig spleen and shown to have a molecular weight of 32kDa. Increased expression of HO-1 can be induced by a large number of structurally unrelated pharmacological and other agents, including cytokines and oxidants, as well as by heat shock and other forms of cellular stress. Baseline levels of HO-1 expression are high in certain tissues, including spleen and liver, but enriched enzyme preparations are recommended for experimental analysis. Recent data have demonstrated a cytoprotective role for HO-1, preventing cell death by modulating intracellular iron levels. |