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Description
PageRuler Unstained Protein Ladder is designed for accurate sizing of proteins in SDS-polyacrylamide gel electrophoresis (1), as well as on PVDF, nylon and nitrocellulose membranes.
PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins ranging in size from 10 kDa to 200 kDa. Each protein in the ladder contains an integral Strep-tag II sequence which can be detected directly on Western blots using a Strep-Tactin-AP* conjugate or an antibody against Strep-tag II (2, 3). Strep-tag II detection systems are not supplied by Fermentas.
Ladder produces sharp bands on SDS-polyacrylamide gel following staining of the gel with PageBlue Protein Staining Solution, PageSilver Silver Staining Kit or by other protein staining methods. PageBlue Protein Staining Solution can also be used to visualize unstained ladders and markers on PVDF membranes.
Content
0.02-0.05 mg/ml of each protein in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol.
Features
Broad range – 10-200 kDa.
Ready-to-use – supplied in a loading buffer for direct loading on gels.
Sharp bands, reference band included in the PageRuler ladder.
Includes a 50 kDa reference band of a greater intensity.
Each protein in the PageRuler ladder contains an integral Strep-tag II sequence.
Applications
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。 |