Description O'RangeRuler 100+500 bp DNA Ladder is designed for precise sizing of PCR products and other double-stranded DNA fragments in agarose gels. It is step ladder with 100 and 500 bp differences between the DNA fragments. It consists of purified and ligated blunt ended basic unit repeats of 100 or 500 bp. Not recommended for DNA quantification. O'RangeRuler 100+500 bp DNA Ladder is supplied with 6X Orange DNA Loading Dye for sample DNA. Quality Control Well-defined bands are formed during agarose gel electrophoresis. The absence of nucleases is confirmed by a direct nuclease activity assay. Features Step ladder with 100 or 500 bp increments. Sharp bands, easy-to-remember fragment sizes. Evenly spaced reference bands (given in red). Ready-to-use – premixed with 6X Orange Loading Dye for direct loading and room temperature storage. Supplied with loading dye for sample DNA. Storage and Loading Buffer 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol. 6X Orange DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. Storage Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C. 注:本产品仅用于生命科学研究实验,不作为任何药物及其他用途。 |