Description Unstained Protein Molecular Weight Marker is designed for accurate sizing of proteins in SDS-polyacrylamide gel electrophoresis (1), as well as on PVDF, nylon and nitrocellulose membranes. It is a mixture of 7 native proteins ranging in size from 14.4 kDa to 116 kDa. Marker produces sharp bands on SDS-polyacrylamide gel following staining of the gel with PageBlue™ Protein Staining Solution, PageSilver™ Silver Staining Kit or by other protein staining methods. PageBlue™ Protein Staining Solution can also be used to visualize unstained ladders and markers on PVDF membranes. Content 0.1-0.2 mg/ml of each protein in 62.5 mM Tris-HCl (pH 7.0 at 25°C), 1 mM EDTA, 2% SDS, 50 mM DTT, 30 mM NaCl, 1 mM NaN3, 0.01% bromophenol blue and 50% glycerol. Features Size range – 14.4-116 kDa. Ready-to-use – supplied in a loading buffer for direct loading on gels. Sharp bands. Applications Accurate protein sizing on SDS-polyacrylamide gels and Western blots. Quality Control Tested in SDS-polyacrylamide gel electrophoresis and Western blotting. 友情提示:本公司产品仅用于生命科学研究实验,不作为任何药物及其他用途。 |