Description MassRuler DNA Ladder Mix is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis. The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments. Features Accurate DNA sizing and quantification–DNA ladder fragments are quantified spectrophotometrically. Sharp bands. Easy-to-remember fragment sizes and quantities. Ready-to-use–premixed with 6X MassRuler DNA Loading Dye for direct loading and room temperature storage. Supplied with loading dye for sample DNA. Quality Control Well-defined bands are formed during agarose gel electrophoresis. The absence of nucleases is confirmed by a direct nuclease activity assay. Storage and Loading Buffer 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol. 6X MassRuler DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol and 60 mM EDTA. Storage Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C. 注:本产品仅用于生命科学研究实验,不作为任何药物及其他用途。 |