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phi29 DNA Polymerase
产地：MBI
货号：EP0091
Applications
Rolling circle amplification (RCA) (6): generation of periodic DNA nanotemplates (7).
Multiple displacement amplification (MDA) (8).
Unbiased amplification of whole genome (WGA, see Fig.1):
– amplification of DNA for SNP (9) and STR (10) detection,
– cell-free amplification of DNA from single cells (11, 12),
– pathogenic organisms or metagenomes (13),
– amplification of DNA from filter paper blood spot samples (14).
DNA template preparation for sequencing.
Protein-primed DNA amplification (15).
In situ genotyping with padlock probes (17).
Recombination based-cloning (18).
Cell-free cloning of lethal DNA (19).
RNA-primed DNA amplification (16).
Description
phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity which allows for highly efficient isothermal DNA amplification (1). phi29 DNA Polymerase also possesses a 3'=>5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA (2) or RNA (3). Therefore 3'-modified primers are highly recommended (4). |