T7 DNA Polymerase产地：MBI 货号：EP0081ApplicationsPurification of covalently closed circular DNA by removal of residual genomic DNA.Primer extension reactions on long templates (1).DNA 3'-end labeling (1).Strand extensions in site-directed mutagenesis (2).Fill-in blunting of 5'-overhang DNA.Second strand synthesis of cDNA (3).In situ detection of DNA fragmentation associated with apoptosis (4).DescriptionT7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'=>3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3'=>5' exonuclease activity towards single- and double-stranded DNA.