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This oligonucleotide probe may be used to detect RNA in formalin-fixed, paraffin-embedded tissues. The technique is simple to perform, employing steps not dissimilar to those used in immunohistochemistry. Tissue sections are dewaxed and rehydrated prior to treatment with proteinase K, followed by incubation with the probe of choice. Bound probe is then detected with an anti-FITC-AP antiserum which is visualized by addition of an enzyme substrate. No specialized equipment is required for in situ hybridization and full instructions are supplied with all in situ hybridization products.
Kappa and lambda mRNA may be detected in normal and neoplastic B cells in human lymphoid tissue. Restriction of either kappa or lambda mRNA denotes monoclonality of lymphoid neoplasms and is useful in distinguishing between neoplastic lymphoid proliferations. Due to the destruction of RNases by formalin fixation, kappa and lampda sequences are conserved in routine surgical tissues. |