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Fucose Maleimide is designed for fucosylating proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Fucose Maleimide require free thiol groups. In the case of proteins, Fucose Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane).
For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag®, 5’ EndTag™ or 3’ EndTag™ nucleic acid labeling systems.
This unique label is ideal for reversible binding of labeled nucleic acid to the matrix, VECTREX® AAL. Fucose labeled nucleic acids can be bound and eluted under mild conditions. Alkaline Phosphatase Aleuria aurantia lectin can be used in dot blot applications to asseess labeling efficiency with the fucose label. |