Dinitrophenyl (DNP) Maleimide is designed for dinitrophenyl labeling of proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Dinitrophenyl Maleimide require free thiol groups. In the case of proteins, Dinitrophenyl Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane). For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag®, 5’ EndTag™ or 3’ EndTag™ nucleic acid labeling systems. |