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NEUROBIOTIN™ Tracer (SP-1120) is an amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for visualizing neural architecture and for the identification of gap junction coupling. Key advantages of NEUROBIOTIN™ Tracer over biocytin and other neuronal labels:
Better solubility
More efficiently iontophoresed
Remains in cell longer
Non-toxic
Can be fixed with formalin or glutaraldehyde
NEUROBIOTIN™ Tracer can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells. It can be delivered by many routes such as intracellular electrodes, microinjection, cutloading, or scrape-loading. NEUROBIOTIN™ Tracer can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods. |