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Biotin (Long Arm) Hydrazide (SP-1100) reacts with aldehyde groups and thus can be used to label carbohydrate groups present in a wide variety of macromolecules. The spacer arm in this product allows optimal accessibility of avidin or streptavidin conjugates to biotin.
Polysaccharides, glycoproteins, or glycolipids. Biotin can be incorporated into viruses, bacteria, or animal and plant cells via their surface carbohydrate groups.
Incorporation of Biotin (Long Arm) Hydrazide occurs onto sugars containing unsubstituted vicinal hydroxyl groups that are usually present at the non-reducing termini of the oligosaccharide.
Coupling of biotin is accomplished in two steps:
(1) A brief treatment of the saccharide with periodate (not included) produces aldehyde groups on the sugars containing unsubstituted vicinal hydroxyl groups.
(2) Biotin (Long Arm) Hydrazide reacts with the aldehyde groups, producing a stable linkage between the saccharide and biotin. |