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Like PTL I, this lectin binds preferentially to galactosides, with N-acetylgalactosamine being the most inhibitory monosaccharide. However, in contrast to PTL I, this lectin prefers the beta anomeric configuration. PTL II shows a high affinity toward blood group H structures and the T-antigen. In general, though, PTL II has lower affinities for inhibiting sugars than does PTL I. PTL I and PTL II also differ in their erythrocyte agglutinating properties. Tissue staining results indicate preference for some vascular elements with overall lighter staining than obtained with PTL I.
This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. |