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This lectin is a dimer of two identical subunits of about 36,000 daltons each with an isoelectric point of about pH 9. Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (a -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (a -1,6) to N-acetylglucosamine or to fucose linked (a -1,3) to N-acetyllactosamine related structures.
This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. |