Cytochrome c Apoptosis Assay Kit Product Overview Kit Summary: • Detection method- Western blotting • Sample type- Cells and Tissues • Species reactivity- Mammalian • Applications- Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol. Features & Benefits: • Simple procedure; takes ~3 hours • Fast and convenient • Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required. Kit components: • Mitochondria Extraction Buffer • 5X Cytosol Extraction Buffer • DTT (1 M) • 500X Protease Inhibitor Cocktail • Anti-Cytochrome c mouse mAb (0.2 mg/ml) Description: Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. BioVision’s Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. Storage Conditions: -20°C Shipping Conditions: gel pack USAGE: For Research Use Only! Not For Use in Humans.