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Mitochondria/Cytosol Fractionation Kit
Product Overview
Kit Summary:
• Detection method- Western blotting, ELISA, or other assays.
• Sample type- Cells and Tissues
• Species reactivity- Mammalian
• Kit size- 25, 100 assays
• Applications- Effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells.
Features & Benefits:
• Simple procedure; takes only 3-4 hours
• Fast and convenient
• The fractionation procedure is simple and staightforward. No ultracentrifugations are required. No toxic chemicals are involved.
Kit components:
• Mitochondria Extraction Buffer
• 5X Cytosol Extraction Buffer
• DTT (1 M)
• Protease Inhibitor Cocktail
Description:
The Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |