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Caspase-7 Antibody (Clone B4-G2)
Product Overview
Antibody Target: Caspase-7
Alternative Name: CASP7,CASP-7,ICE-LAP3,CMH-1,MCH3, EC 3.4.22.60
Host: Mouse
Antibody Type: Monoclonal (Clone B4-G2)
Isotype: Mouse IgG1
Immunogen: Synthetic peptide from human caspase-7.
Accession#: BAG61059
Gene ID: 840
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 µg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM thimerosal and 50% glycerol
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot, Immunoprecipitation
Application & Usage: Western blot analysis (0.5-2 µg/ml) and immunoprecipitation (4-10 µg/ml). However the optimal conditions should be determined individually. Detects both pro-caspase-7 (34 kDa) and the large subunit of cleaved caspase-7 (19 kDa) in apoptosis cells on SDS-PAGE immunoblots.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: The caspase family of cysteine proteases plays a key role in apoptosis. Caspase-7, also known as Mch3α, ICE/LAP3 and CMH1, is a 34 kDa protein that is activated during Fas- and TNF-induced apoptosis. Caspase-7 has been identified as a major contributor to the execution phase of cellular apoptosis. It cleaves essential cellular machinery following its own cleavage from an inactive proenzyme to an active heterodimeric form. Caspase-7 is processed by caspase-3, -6, -8, -9, and –10 as well as by Granzyme B.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |