Components:
Equilibration Buffer 90416 3.0 mL -15°C to -25°C
Reaction Buffer 91417 2.0 mL -15°C to -25°C
TdT Enzyme 90418 0.672 mL -15°C to -25°C
Stop/Wash Buffer 90419 20 mL -15°C to -25°C
Anti-Digoxigenin-Peroxidase* 90420 3.0 mL 2°C to 8°C
Plastic Coverslips 90421 100 ea. Room Temp.
Control Slides 90422 2 each Room Temp.
DAB Substrate 90423 130 μL 2°C to 8°C
DAB Dilution Buffer 90424 6.5 mL 2°C to 8°C
*affinity purified sheep polyclonal antibody
Number of samples per kit: Sufficient materials are provided to stain 40 tissue specimens of approximately 5 cm2 each when used according to instructions. Reaction Buffer will be fully consumed before other reagents when kits are used for slide-mounted specimens.
Product Overview:
The ApopTag® Plus Peroxidase In Situ Apoptosis Detection Kit detects apoptotic cells by labeling and detecting DNA strand breaks by the indirect TUNEL method. The kit provides sufficient reagents for immunoperoxidase staining of 40 samples, and includes ApopTag(R) Positive control Slides and DAB buffer and substrate. Results are visualized using brightfield microscopy.
The ApopTag® Peroxidase Kits have been qualified for use in histochemical and cytochemical staining of the following specimens: formalin-fixed, paraffin-embedded tissues, cryostat sections, cell suspensions, cytospins, and cell cultures. Whole mount-methods have been developed (34, 45). (See datasheet Sec. V. for References).
The staining specificity of the ApopTag® Peroxidase Kits has been demonstrated by Chemicon and many other laboratories. Chemicon has tested many types of model cell and tissue systems, including: (a) human prostate, thymus, and large intestine (in-house data); (b) rat ventral prostate post-castration (21), (c) rat thymus lymphocytes treated in vitro with dexamethasone (3, 13), (d) 14-day mouse embryo limbs (1) and (e) rat mammary gland in regression after weaning (36). In the thymocyte and prostate models, agarose gel electrophoresis was used to assess the amount of DNA laddering, which peaked coincidentally with the maximum percentage of stained cells. Numerous journal publications from laboratories worldwide have established the usefulness of ApopTag® Kits. (See datasheet Sec. V. References, Publications Citing ApopTag® Kits).