TUNEL法检测DNA片断化（荧光法）
MERCK相关产品资料：
QIA39 FragEL™ DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme ( FragEL™ DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme )
TUNEL Assay
Allows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population. Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL™ DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.
产品信息
Format Flow cytometry or fluorescence microscopy
Form 50 Tests
Detection method Fluorescence
Species reactivity a broad range of species
Applications Flow Cytometry
Avoid freeze/thaw Yes
Assay time 2 h
Sample type Frozen or paraffin sections, fixed cell preparations or cell suspensions
Kit contains Proteinase K, Euilibration and Lbeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.
Store and ship information
<b>Hazardous Materials Attention:</b> Due to the nature of the Hazardous Materials in this shipment, additional shipping charges <i>may</i> be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS