检测原理:
通过DNA 片段末端标记(Fragment End Labeling, FragELTM)检测凋亡细胞的DNA 片段。细胞凋亡中,
染色体DNA 双链断裂或单链断裂而产生大量的粘性3'-OH 末端,可在脱氧核糖核苷酸末端转移酶
(TdT)的作用下,将生物素标记和未标记的脱氧核糖核苷酸标记到DNA 的3'-末端,生物素标记的脱
氧核糖核酸可以通过Streptavidin-HRP 进行检测,通过DAB 显色,从而检测到凋亡细胞DNA 片段。由
于正常的或正在增殖的细胞几乎没有DNA 的断裂,因而没有3'-OH 形成,很少能够被染色。以甲基绿
复染,便于从形态学上分辨评估正常细胞和凋亡细胞。
Product information
Format: Microscopy
Form: 50 Tests
Detection method: Colorimetric
Species reactivity: a broad range of species
Applications: Frozen Sections
Paraffin Sections
Assay time :4 h
Sample type : Frozen or paraffin sections or fixed-cell preparations
Kit contains : Counterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H2O2/Urea Tablets, Methyl Green Counterstain, and a user protocol.
Storage -20°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Safety information
S Phrase S: 26-36/37/39-45-53
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Avoid exposure - obtain special instructions before use.
R Phrase R: 23/25-36/37/38-42/43-45-61
Toxic by inhalation and if swallowed.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
May cause cancer.