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CD14
Coding sequence in expression plasmid
Membrane-bound form
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein which acts as a bacterial pattern recognition receptor[1]. CD14 is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2[2]. CD14 binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections[3].
1. Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity, 1(6):509-16.
2. Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem, 276(24): 21129-35.
3. Aderem A. & R.J. Ulevitch, 2000. Toll-like receptors in the induction of the innate immune response. Nature, 406(6797):782-7.
This gene is available in pUNO1 expression plasmid selectable with Blasticidin.
Human CD14 (pUNO1-hCD14)
ORF Size: 1128 bp
Subclone: NcoI - EcoRI
Mouse CD14 (pUNO1-mCD14)
ORF Size: 1101 bp
Subclone: NcoI - NheI
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