|
MD2
Coding sequence in expression plasmid
Alias: Ly96
Lymphocyte antigen 96 / Myeloid differentiation factor 2
MD-2, a 25-kDa secreted protein, is an accessory molecule required for efficient LPS-induced signaling through TLR4. MD-2 binds to both LPS and the extracellular domain of TLR4. Following synthesis, MD-2 is either secreted directly into the medium as a soluble, active protein, or binds directly to TLR4 in the endoplasmic reticulum before migrating to the cell surface. The secreted form of MD-2 forms a stable complex with LPS, in a CD14-dependent process [2]. The MD-2/LPS complex activates TLR4 in the absence of CD14 or free LPS. indicating that the activating ligand of TLR4 is the MD-2/LPS complex. MD-2 also increases TLR2 responsiveness to TLR2 ligands [3].
1. Shimazu R. et al., 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med, 189(11):1777-82.
2. Kennedy MN. et al., 2004. A complex of soluble MD-2 and lipopolysaccharide serves as an activating ligand for Toll-like receptor 4. J Biol Chem. 2004 Aug 13;279(33):34698-704.
3. Dziarski R. et al., 2001. MD-2 enables Toll-like receptor 2 (TLR2)-mediated responses to lipopolysaccharide and enhances TLR2-mediated responses to Gram-positive and Gram-negative bacteria and their cell wall components. J Immunol, 166(3):1938-44.
This gene is available in pUNO1 expression plasmid selectable with Blasticidin.
Bovine MD2 (pUNO1-bMD2)
Genbank: NM_001046517.1
ORF Size: 483 bp
Subclone: BspLU11I - NheI
Human MD2 isoform 1 (pUNO1-hMD2a)
ORF Size: 483 bp
Subclone: BspLU11I - NheI
Human MD2 isoform 2 (pUNO1-hMD2b)
Genbank: NM_001159711.1
ORF Size: 393 bp
Subclone: BspLU11I - NheI
Mouse MD2 isoform 1 (pUNO1-mMD2a)
Genbank: NM_016923.2
ORF Size: 483 bp
Subclone: BspLU11I - NheI
|