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SIRT5 (GST-tagged), Human recombinant
Intracellular regulatory proteins with mono-ADP-ribosyltransferaseﾠactivity.
Product Overview
Product Name: SIRT5 (GST-tagged), Human recombinant
Alternate Name/Synonyms: NAD-dependent Deacetylase 5; SIR2-like Protein 5; SIR2L5; Sirtuin 5; Silent Information Regulator 5
Gene Symbol: SIRT5
Accession #: Q9NXA8
Gene ID: 8359
Source: E. coli
Appearance: Liquid
Physical Form Description: 50 mM sodium phosphate, pH 7.2, 100 mM sodium chloride, and 20% glycerol.
Molecular Weight: 60.6 kDa (NT GST Tag), 26 kDa (native)
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry ice
Background Information: The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. There are seven human sirtuins, which have been designated SIRT1-7. SIRT5 is located in the mitochondrial matrix and its functions are largely still being elucidated, however a few promising substrates have been studied. SIRT5 has been shown to deacetylate carbamoyl phosphate synthetase 1 (CSP1), activating the enzyme to catalyze the first step of the urea cycle. CSP1 is important in the detoxification of excess ammonia that can accumulate during fasting. Cytochrome C, another mitochondrial enzyme involved in oxidative metabolism can also be deacetylated by SIRT5. SIRT5 is predominantly found in the heart, brain, testis, and lymphoblasts.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |