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Histone H4 (1-103 aa), Human recombinant
Core component of nucleosome
Product Overview
Product Name: Histone H4 (1-103 aa), Human recombinant
Alternate Name/Synonyms: Histone H4, H4
Gene Symbol: HIST1H4A
Accession #: P62805
Gene ID: 8359
Source: E. coli
Appearance: Lyophilized powder
Physical Form Description: Lyophilized powder. Recommended buffer is 50 mM NaPO₄ containing 100 mM sodium chloride and 20% glycerol.
Molecular Weight: 11.5 kDa (1-136 aa)
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry ice
Background Information: H2A is a core component of nucleosome. A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around an octamer of histones. The octamer is composed of two of each histone H2A, H2B, H3, and H4. DNA accessibility is regulated via a complex set of post-translational modifications of these histones, also called histone code, and nucleosome remodeling. Histones H2A and H2B form a dimer. Histones H3 and H4 form a tetramer. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core. Histone H4 undergoes many modifications which include acetylation, phosphorylation and methylation that are important for regulation of gene transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |