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DOT1L (2- 416 aa), Human recombinant
Involved in transcriptional activation, DNA damage repair, and cell cycle regulation.
Product Overview
Product Name: DOT1L (2- 416 aa), Human recombinant
Alternate Name/Synonyms: Dot1-like; Disruptor of Telomeric Signaling 1-Like
Gene Symbol: DOT1L
Accession #: Q8TEK3
Gene ID: 84444
Source: E. coli
Appearance: Liquid
Physical Form Description: 20 mM Tris, pH 8.0, containing 200 mM sodium chloride, 1 mM EDTA and 1 mM DTT.
Molecular Weight: 74.1 kDa (2- 416 aa + NT GST Tag)
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry ice
Background Information: Dot1L is a non-SET domain containing methyltransferase. It is the only enzyme known to methylate histone 3 at lysine 79, where it catalyzes mono-, di-, and trimethylation. Proper Dot1L function is necessary for transcriptional activation of many genes, DNA damage repair, and cell cycle regulation. Dot1L plays an essential role in MLL-rearranged leukemias and is being investigated as a drug-target for these leukemias. Experiments using siRNA or shRNA have shown that knockdown of Dot1L leads to loss of proliferation in lung cancer cells or faster reprogramming and increased yield of induced pluripotent stem cells, respectively. This protein product contains the methyltransferase domain of Dot1L.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |