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PCAF bromodomain (714-831 aa) (GST-tagged), Human recombinant
A bromodomain promoting transcription of viral proteins
Product Overview
Product Name: PCAF bromodomain (714-831 aa) (GST-tagged), Human recombinant
Alternate Name/Synonyms: p300/CBP-associated factor; KAT2B
Gene Symbol: KAT2B
Accession #: Q92831
Gene ID: 8850
Source: E. coli
Appearance: Liquid
Physical Form Description: 50 mM Tris, pH 8.0, containing 150 mM sodium chloride and 20% glycerol.
Molecular Weight: 40.9 kDa (714-831 aa + NT GST Tag)
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry ice
Background Information: The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. p300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |