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BRG1 bromodomain (1448-1575 aa) (GST-tagged), human recombinant
Activates genes normally repressed by chromatin
Product Overview
Product Name: BRG1 bromodomain (1448-1575 aa) (GST-tagged), human recombinant
Alternate Name/Synonyms: Protein BRG-1, BAF190A Mitotic growth and transcription activator, BRG1-associated factor 190A, ATP-dependent helicase SMARCA4.
Gene Symbol: SMARCA4
Accession #: P51532
Gene ID: 6597
Source: E. coli
Appearance: Liquid
Physical Form Description: 50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5% glycerol, and 5 mM β-mercaptoethanol.
Molecular Weight: 41.8 kDa (1448-1575 aa + GST Tag)
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry ice
Background Information: The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRG1 is a member of the SWI/SNF protein family, which forms part of a large ATP-dependent chromatin remodeling complex. This complex is required for transcriptional activation of genes normally repressed by chromatin. BRG1 is mutated in many cancer cell lines, such as breast, prostate, lung, pancreas and colon. Further, BRG1 has an important role as a tumor suppressor. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |