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Human CellExp™ IL-1 Alpha, Human Recombinant
Plays one of the central roles in the regulation of the immune responses
Product Overview
Product Name: IL-1 Alpha, Human CellExp™, human recombinant
Alternate Name/Synonyms: IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin
Gene Symbol: IL-1a
Accession #: P01583
Gene ID: 3552
Source: HEK 293 cells
Appearance: Lyophilized powder
Physical Form Description: Lyophilized from 0.22 μm filtered solution in in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight: Calculated MW of 18.0 kDa with no tag. DTT-reduced protein migrates as 22 kDa. in SDS-PAGE
Purity by SDS-PAGE: ≥97%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg) by LAL method
Biological Activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 <1pg/ml, corresponding to a specific activity of >1X10^9 Unit/mg.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA, IL1F1, and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |