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Human CellExp™ DKK3, human recombinant
Proposed as tumor suppressor gene and a marker for tumor blood vessels
Product Overview
Product Name: Human CellExp™ DKK3, human recombinant
Alternate Name/Synonyms: DKK3, REIC, RIG
Gene Symbol: DKK3
Accession #: Q9UBP4
Gene ID: 27122
Source: HEK 293 cells
Appearance: Lyophilized powder
Physical Form Description: Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 37.6 kDa. The predicted N-terminus is Pro 23. DTT-reduced Protein migrates as 50-60 kDa due to glycosylation.
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Biological Activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 2.0-11.4 μg/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Members of the Dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumor suppressor gene and a marker for tumor blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |