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InSolution™ TGF-β RI Kinase Inhibitor VI, SB431542
A cell-permeable triarylimidazole compound that is shown to effectively inhibit cellular Smad2 phosphorylation (>90% inhibition by 10 µM inhibitor) upon vector-mediated expression of constitutively active ALK4, ALK5, or ALK7 in NIH 3T3 cells, while exhibiting little effect against Smad1 phosphorylation by other members of type I receptors for TGF-β in NIH 3T3 cultures expressing active ALK1, 2, 3, or 6. When tested directly in cell-free kinase assays, SB431542 is demonstrated to potently inhibit the activity of ALK4 and ALK5 (IC50 = 140 nM and 94 nM, respectively) with no or much reduced potency toward a panel of 24 other kinases (IC50 ≥10 µM in the presence of 10 µM ATP), including ALK2 and ALK6. Reported to improve the efficiency of 4-TF-induced human iPSCs generation from fibroblast cultures by >200-fold when used together with PD0325901 (Cat. No. 444966) and Thiazovivin (Cat. No. 420220). The solid form of this compound (Cat. No. 616461) is also available.
产品信息
Form Liquid
Formulation A 100 mM (5 mg/119 µl) solution of SB431542 (Cat. No. 616461) in DMSO.
Molar mass 420.4
Protect from Light 是的
Hygrocopic 是的
Packaged under inert gas 是的
Purity ≥97% by HPLC
Chemical formula C22H16N4O3 • 2H2O
CAS number 301836-41-9
商店和运输信息
Storage -20°C
Ship Blue Ice Only
Irritant |