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Leukotriene B4 Express EIA Kit
Cayman Chemical Item Number 10009292
Description Limit of detection:80% B/B0: 45 pg/ml · Sensitivity:50% B/B0: 150 pg/ml · LTB4 stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. Plasma levels of LTB4 increase from <100 pg/ml to >100 ng/ml following leukocyte stimulation. LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 and then further oxidized to ω-carboxy dinor LTB4 and ω-carboxy tetranor-LTB3. LTB4 is not excreted in the urine. Cayman’s LTB4 Express EIA Kit is a competitive assay that can be used for quantification of LTB4 in plasma and other sample matrices. The EIA typically displays an IC50 (50% B/B0) of approximately 150 pg/ml and a detection limit (80% B/B0) of approximately 45 pg/ml.
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Leukotriene B4 100%
20-hydroxy Leukotriene B4 260%
20-carboxy Leukotriene B4 13.4% |