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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for Caco-2 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for Caco-2 Cell is pre-optimized for transfecting Caco-2 cells. The Caco-2 cell line is an immortalized line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh. The Caco-2 cell line is widely used with in vitro assays to predict the absorption rate of candidate drug compounds across the intestinal epithelial cell barrier. The assay requires that drug absorption rates be determined 21 days after Caco-2 cell seeding to allow for monolayer formation and cell differentiation. Caco-2 cells may also refer to a cell monolayer absorption model. Cell-based functional assays, such as the Caco-2 drug transport model for assessing intestinal transport, are extremely valuable for screening lead compounds in drug discovery.
最佳转染条件:
Confluency of cell on the day of shaving 90%
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 65%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |