GenJet (Ver. II) DNA In Vitro Transfection Reagent for LNCaP Cell

产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for LNCaP Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for LNCap is pre-optimized for transfecting LNCap cells. LNCap cells are a cell line of human cells commonly used in the field of oncology. LNCaP cells are androgen-sensitive human prostate adenocarcinoma cells derived from the left supraclavicular lymph node metastasis from a 50-year-old caucasian male in 1977. They are adherent epithelial cells growing in aggregates and as single cells.
最佳转染条件:
Confluency of cell on the day of shaving 95%
GenJet? (μl) : DNA (μg) Ratio   3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Maximal Efficiency     48 hours
Transfection Results:
Reporter Gene   EGFP
Plasmid    pEGFP-N3 (CMV promoter)
Efficiency (GFP %)  80%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.