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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Kit for HUVEC
功能:
GenJet™ DNA In Vitro Tranfection Reagent for HUVEC is pre-optimized for transfecting human umbilical vein endothelial cells (HUVEC). HUVEC is one of hard-to-transfect cell lines partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 60% efficiency. To achieve maximal efficiency, the "Shaved Cell Transfection" protocol was optimized for transfecting HUVEC. Refer to the following optimal transfection conditions for maximal transfection efficiency on HUVEC. GenJet™ reagent, 1.0 ml, is sufficient for 300 transfections in 24 well plates or 150 transfections in 6 well plates.
组份:
1x1.0 ml of GenJet™ DNA Transfection Reagent
1x1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail
最佳转染条件:
Confluency of cell on the day of shaving 95~100%
GenJet™ (µl) : DNA (µg) Ratio 4:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 56%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |