GenJet (Ver. II) DNA In Vitro Transfection Reagent for 3LL Cell

产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for 3LL Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for 3LL cell is pre-optimized for transfecting 3LL cells. 3LL also called Lewis lung carcinoma was discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass.
最佳转染条件:
Confluency of cell on the day of shaving 80%
GenJet™ (µl) : DNA (µg) Ratio   3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency     48 hours
Transfection Results:
Reporter Gene   EGFP
Plasmid    pEGFP-N3 (CMV promoter)
Efficiency (GFP %)  85%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.