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产品名称:GenJet In Vitro DNA Tranfection Reagent for Primary Macrophages
功能:
GenJet™ DNA In Vitro Tranfection Reagent for Primary Macrophages is pre-optimized for transfecting primary macrophagess. Macrophages are white blood cells within tissues, produced by the differentiation of monocytes. Monocytes and macrophages are phagocytes, acting in both non-specific defense (innate immunity) as well as to help initiate specific defense mechanisms (adaptive immunity) of vertebrate animals. Their role is to phagocytose (engulf and then digest) cellular debris and pathogens either as stationary or as mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen. They can be identified by specific expression of a number of proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), Lysozyme M, MAC-1/MAC-3 and CD68 by flow cytometry or immunohistochemical staining.
最佳转染条件:
Confluency of cell on the day of shaving 95%
Cell density for plating 1.3x10^5 cells per square centimeter
GenJet™ (µl) : DNA (µg) Ratio 4:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 75%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |