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Arachidonoyl m-Nitroaniline
Cayman Chemical Item Number 90059
AmNA (CAS 1175954-87-6)
Description
Arachidonoyl m-Nitroaniline (AmNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1↗ FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its nominal endogenous substrate anandamide (arachidonyl ethanolamide; AEA). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate (see also Decanoyl m-Nitroaniline; Item No. 90349). Exposure of AmNA to FAAH activity results in the release of the yellow colorimetric dye m-nitroaniline (ε = 13,500 at 410 nm). This offers the potential for fast and convenient measurements of FAAH activity using a 96 well plate spectrophotometer.
Synonyms AmNA
Formal Name N-(3-nitrophenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide
CAS Number 1175954-87-6
Molecular Formula C26H36N2O3
Formula Weight 424.6
Formulation A solution in methyl acetate
Purity ≥98%
λmax 203, 242 nm
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)Nc1cccc(c1)[N](=O)O
InCHI Code 1S/C26H36N2O3/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-22-26(29)27-24-20-19-21-25(23-24)28(30)31/h6-7,9-10,12-13,15-16,19-21,23H,2-5,8,11,14,17-18,22H2,1H3,(H,27,29)/b7-6-,10-9-,13-12-,16-15-
InCHI Key OITDPRAMDXERDT-DOFZRALJSA-N |