Poly(I:C) (HMW) Fluorescein Fluorescein-labeled poly(I:C) (HMW) Poly(I:C) Fluorescein was chemically labeled by covalent coupling of a fluorescein probe containing a reactive alkylating group. This confers fluorescent properties to poly(I:C) (HMW) whilst retaining TLR3 recognition properties. Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection. Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production. Poly(I:C) is recognized by Toll-like receptor 3 (TLR3). Specificity: TLR3 agonist Working Concentration: 10 ng - 10 μg/ml Endotoxin level: EndoFit™ (<0.001 EU/μg) Appearance: Pale yellow lyophilized powder Spectral Properties of Fluorescein Excitation λ max: 492 nm Emission λ max: 518 nm