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LPS-EB Biotin
Biotinylated LPS from E. coli O111:B4
LPS-EB Biotin is biotinylated ultrapure lipopolysaccharide (LPS) from E. coli 0111:B4. LPS is recognized by toll-like receptor 4 (TLR4) which interacts with three different extracellular proteins: LPS binding protein (LBP), CD14 and, myeloid differentiation protein 2 (MD-2), to induce a signaling cascade leading to the activation of NF-κB and the production of proinflammatory cytokines.
However, most LPS preparations on the market are contaminated by other bacterial components, such as lipoproteins, thus activating TLR2 signaling as well as TLR4 signaling. The ultrapure preparation that InvivoGen provides only activates the TLR4 pathway.
LPS-EB Biotin can be used for the detection of LPS binding sites by cytometry or fluorescence microscopy.
Specificity: TLR4 agonist
Working concentration: 10 ng - 10 μg/ml
Endotoxin level: 1 x 106 EU/mg |