5′ppp-dsRNA 5' triphosphate double stranded RNA (5' ppp-dsRNA) 5’triphosphate double stranded RNA (5’ ppp-dsRNA) is a synthetic ligand for retinoic acid-inducible protein I (RIG-I). RIG-I is a cytosolic pattern recognition receptor that senses pathogen-associated molecular patterns (PAMPs) on viral RNA and triggers an antiviral immune response by the activation of type-I interferons (IFN-α and -β). 5’ ppp-dsRNA is also available complexed with the cationic lipid transfection reagent LyoVec™ to facilitate its uptake. Specificity: RIG-I agonist Working Concentration: 300 ng- 1 μg/ml Endotoxin level: EndoFit™ (<0.001 EU/µg) Solubility: 0.1 mg/ml in water Formulation: naked 5’ppp-dsrna 5’- pppGCAUGCGACCUCUGUUUGA -3’ (19 mer) 3’- CGUACGCUGGAGACAAACU -5’ (19 mer) 5’ triphosphate double stranded RNA is obtained by hybridization of one 5’ triphosphate single-stranded 19-mer phosphodiester RNA with its complementary strand (non-triphosphatase). Each strand is chemically synthesized by solid-phase synthesis and purified by reverse phase HPLC.
