ODN 2395 FITC FITC labeled CpG oligonucleotide - Human / Mouse TLR9 ligand CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. They have been shown to induce a coordinated set of immune responses based on the activation of immune cells primarily involved in the recognition of these molecules. ODN 2395 FITC is a C-class CpG ODN specific for human / mouse TLR9. ODN 2395 FITC can be used to evaluate CpG ODN cellular uptake and localization by confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm) or flow cytometry. Specificity: human / murine TLR9 agonist Working concentration: 10 ng -10 μg/ml Quality control - Endotoxin levels <0.001 EU/µg (measurement by kinetic chromogenic LAL assay). - Human TLR9 (hTLR9) activity is tested using HEK-Blue™ hTLR9 cells. ODN 2395 sequence 5’-tcgtcgttttcggcgc:gcgccg-3’ (22 mer) Note: Bases are phosphorothioate (nuclease resistant), palindrome is underlined. Spectral Properties of FITC Excitation λ max: 495 nm Emission λ max: 520 nm
