ODN 2336 FITC FITC labeled CpG oligonucleotide - Human TLR9 ligand CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. They have been shown to induce a coordinated set of immune responses based on the activation of immune cells primarily involved in the recognition of these molecules. ODN 2336 FITC is a A-class CpG ODN specific for human TLR9. ODN 2336 FITC can be used to evaluate CpG ODN cellular uptake and localization by confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm) or flow cytometry. Specificity: human TLR9 agonist Working concentration:10 ng -10 μg/ml ODN 2336 sequence 5’- gggGACGAC:GTCGTGgggggg -3’ (21 mer) Note: Bases in capital letters are phosphodiester, those in lower case are phosphorothioate. Palindrome is underlined. Spectral Properties of FITC Excitation λ max: 495 nm Emission λ max: 520 nm
