ODN 2216 Class A CpG oligonucleotide - Human TLR9 ligand CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects. ODN 2216 is a A-class CpG ODN specific for human TLR9. Class A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string. They induce high IFN-α production from plasmacytoid dendritic cells (pDC) but are weak stimulators of TLR9-dependent NF-κB signaling. ODN 2216 is also available on ready-to-use 8-well strips pre-coated at 7 different concentrations and a negative control. Specificity: human TLR9 agonist Working concentration: 5 µM (10 μg/ml) Solubility: 5 mg/ml in water ODN 2216 sequence 5’-ggGGGACGA:TCGTCgggggg-3’ (20 mer) Note: Bases shown in capital letters are phosphodiester, and those in lower case are phosphorothioate (nuclease resistant). Palindrome is underlined. Quality control - TLR9 activity is tested using HEK-Blue™ TLR9 cells. - The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
