ODN 2007 Class B CpG oligonucleotide - Bovine / porcine TLR9 ligand CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects. ODN 2007 is a B-class CpG ODN specific for bovine / porcine TLR9. Specificity: bovine / porcine TLR9 agonist Working concentration: 5 µM (10 μg/ml) Solubility: 5 mg/ml in water ODN 2007 sequence 5’- tcg tcg ttg tcg ttt tgt cgt t -3’ (22 mer) Note: Bases are phosphorothioate (nuclease resistant). Quality control - The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
