ODN 2006 (ODN 7909) Class B CpG oligonucleotide - Human TLR9 ligand CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects. ODN 2006 (ODN 7909) is a B-class CpG ODN specific for human TLR9. ODN 2006 is also available on ready-to-use 8-well strips pre-coated at 7 different concentrations and a negative control. Synonyms: ODN 7909, PF_3512676 Specificity: human TLR9 agonist Working concentration: 5 µM (10 μg/ml) Solubility: 5 mg/ml in water ODN 2006 sequence 5’-tcgtcgttttgtcgttttgtcgtt-3’ (24 mer) Note: Bases are phosphorothioate (nuclease resistant). Quality control - TLR9 activity is tested using HEK-Blue™ TLR9 cells. - The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
