ODN 1585 Class A CpG oligonucleotide - Murine TLR9 ligand CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects. ODN 1585 is a CpG ODN class A specific for mouse TLR9. Class A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string. They induce high IFN-a production from plasmacytoid dendritic cells (pDC) but are weak stimulators of TLR9-dependent NF-κB signaling. Specificity: murine TLR9 agonist Working concentration: 5 µM (10 μg/ml) Solubility: 5 mg/ml in water ODN 1585 sequence 5’- ggGGTCAACGTTGAgggggg -3’ (20 mer) Note: Bases in capital letters are phosphodiester and those in lower case are phosphorothioate. Quality control - TLR9 activity is tested using HEK-Blue™ TLR9 cells. - The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.