pFUSE-mIgG2Ae1-Fc pFUSE-mIgG2Ae1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG2 heavy chain mutated L235E + E318A/K320A/K322A and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. pFUSE-mIgG2Ae1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted. Isotype : IgG2a mouse Mutations: L235E + E318A/K320A/K322A Characteristics: - Reduced binding to FcγRI and C1q - Reduced ADCC and CDC Both plasmids are selectable with Zeocin™.