
Description: Millicell μ-Migration Assay Kit Trade Name: Millicell Qty/Pk: 1 Kit Product Overview: Cell migration occurs in a variety of biological processes including wound healing, embryonic development, and immune response. Because of the importance of cell migration in angiogenesis, oncology, neurology, and especially immunology, there is an increased interest in understanding the underlying biological mechanisms at a single cell level. Traditional migration assays employ a multiwell or Boyden chamber in which cells are seeded on top of an insert in serum-free medium, while serum or similar chemoattractants are placed in the well below. Limitations to multiwell migration assays include the following: the gradients are not linear, well-established, or controlled, the quantification of migratory cells is based on an endpoint measurement rather than a dynamic determination, and imaging of the cells while they are migrating is currently not possible. Millipore's Millicell µ-Migration Assay Kit overcomes the limitations of traditional multiwell migration assays. The innovative design of the µ-Migration slide promotes a stable diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours. Made from a plastic with high optical qualities similar to those of glass, the µ-Migration Slide is designed for video microscopy assays. At specific time intervals, images of the observation area can be acquired, allowing real-time monitoring and quantitative measurements of cell migration. « Collapse Key Applications: Track single cell and cell population migration (migration assay, cell culture) Species Reactivity: Human Rat Mouse Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Components: 1. Millicell® µ-Migration Slides: (Part No. CS204500) Four uncoated sterilized slides. Each slide contains 3 chambers. 2. Millicell µ-Migration Caps and Plugs: (Part No. CS204501) Forty-eight of each. Twelve caps and twelve plugs for each slide. 3. Human Collagen IV: (Part No. CS204498) One 100 µg vial of 1 mg/mL Human Collagen IV. 4. Acetic Acid Buffer: (Part No. CS204485) One vial containing 1.5 mL of 0.5 M acetic acid. 5. BSA Solution: (Part No. CS204507) One vial containing 100 µL of 30% BSA solution. 6. Beveled pipette tips: Fisherbrand® SureOne™ 200 µL tips, Fisher Cat. No. 02-707-419. Two 96-tip boxes. Storage Conditions: 1. Millicell® µ-Migration Slides (Part No. CS204500). Store at room temperature. 2. Millicell µ-Migration Caps and Plugs (Part No. CS204501) Store at room temperature. 3. Human Collagen IV (Part No. CS204498) Store at -20ºC. 4. Acetic Acid Buffer (Part No. CS204485) Store at room temperature. 5. BSA Solution (Part No. CS204507) Store at -20ºC. 6. Beveled Pipette Tips. Store at room temperature.View All » Product Name: Millicell® μ-Migration Assay Kit Materials Required but Not Delivered: 1. Adherent cells 2. Inverted Microscope (phase contrast, fluorescence, etc.) 3. Heating stage 4. Time-lapse video equipment (CCD camera, video camera, acquisition software) 5. Motorized stage and autofocus (x,y,z) (optional) |