|
Cell Proliferation ELISA, BrdU (colorimetric)
Application
The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:
•Detection and quantification of cell proliferation induced by growth factors and cytokines
•Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
•Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
•Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
Benefits
•Safe: No radioactive isotopes are used
•Accurate: Results obtained strongly correlate to the number of proliferating cells (low mean deviation)
•Sensitive: Chemiluminescence technology provides sensitivity that is at least equivalent to the [3H]-thymidine incorporation assay
•Fast: Fixation and denaturation in a single step, one washing and two incubation steps only; the use of a multi-well ELISA reader allows a larger number of samples to be processed
•Convenient: Reagents are provided in a stable and optimized form; the entire assay is performed in one microplate; mild fixation and DNA denaturation preserve cellular morphology
•Function-tested: Every lot is function-tested on proliferating cells in comparison to a master lot
Product Description
Sample material: Adherent and suspension cells cultured in 96-well microplates
Background Information
Cellular proliferation requires the replication of genomic DNA. Thus, monitoring DNA synthesis is an indirect parameter of cell proliferation, as well as being suitable for the study of the regulation of DNA synthesis. [3H]-thymidine has traditionally been used to label the DNA of replicating (cycling) cells. To circumvent the disadvantages associated with the use of the [3H]-thymidine, nonradioactive alternatives based on 5-bromo-2'-deoxyuridine (BrdU) have been developed. The use of chemiluminescence technology provides enhanced sensitivity and a broad measurement range.
Contents
1.BrdU Labeling Reagent
2.FixDenat , ready-to-use
3.Anti-BrdU-POD
4.Antibody Dilution Solution, ready-to-use
5.Washing Buffer PBS, 10x conc.
6.Substrate Solution TMB, ready-to-use |